Definitive qPCR: Nucleic acid quality control
The assessment of nucleic acid purity and integrity is one of the essential steps preceding microarray, qPCR and sequencing experiments. Both quality parameters have direct effects on the reliability, accuracy and biological/clinical relevance of results obtained using these molecular techniques. This book discusses both provides a detailed overview of the important evaluation criteria that make up successful nucleic acid quality control, with the main focus on RNA. It traces the historical context of nucleic acid extraction, describes prokaryotic and eukaryotic in vivo RNA degradation mechanisms, summarises information about the different instruments used to assess nucleic acid quality and analyses RNA and DNA purity as well as RNA integrity in detail. The appendices contain a general illustration of some of the molecular methods that rely on high quality nucleic acids and a set of figures making the case for MIQE, the guidelines laid out for qPCR assay design and publication. The book is extensively referenced and hyperlinked to additional sources of information. It is ideal for anyone wanting to find all relevant information about nucleic acid quality assessment in one convenient place.
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